Mai ka hoʻomaka ʻana o ka maʻi maʻi, ua hana koʻikoʻi ka hoʻāʻo diagnostic i ka pale ʻana i ka hoʻolaha ʻana o SARS-CoV-2, ka maʻi i hoʻokumu ʻia.COVID-19.Nā hoʻāʻo antigen wikiwikihana ʻia ma ka home a i ʻole ma kahi hoʻokolohua lapaʻau e hāʻawi i nā hopena i loko o 15 mau minuke a i ʻole.ʻO ka maʻi mua o ke kanaka, ʻoi aku ka wikiwiki e ʻimi i ka lāʻau lapaʻau a hoʻokaʻawale iā lākou iho mai nā poʻe ʻē aʻe.Akā ke ʻike ʻia nā ʻano hou o ka maʻi, ʻaʻole ʻike ʻia kēlā mau ʻano e kēia mau hoʻāʻo.
Hoʻolālā ʻia ka hapa nui o nā hoʻokolohua antigen e ʻike i ka protein nucleocapsid SARS-CoV-2 a i ʻole N-protein.Loaʻa kēia pūmua i ka nui i loko o nā ʻāpana viral a me nā poʻe maʻi.pahu hoʻāʻo wikiwikiLoaʻa maʻamau i ʻelua mau ʻano antibodies diagnostic e hoʻopaʻa i nā ʻāpana like ʻole o ka protein N.Ke hoʻopaʻa ʻia kahi antibody i ka protein N i loko o kahi laʻana, ʻike ʻia kahi laina kala a i ʻole kekahi hōʻailona ʻē aʻe ma ka pahu hoʻāʻo, e hōʻike ana i kahi maʻi.
ʻO ka Protein N he 419 amino acid structural units.Hiki ke pani ʻia kekahi o lākou e kekahi amino acid e ka mutation.ʻO ka hui noiʻi i alakaʻi ʻia e Ph.D.Ua hoʻomaka ʻo Philip Frank lāua ʻo Eric Ortlund o ke Kulanui ʻo Emory e noiʻi i ke ʻano o ka hoʻololi ʻana o kēia ʻakika amino hoʻokahi i ka hana o kahi hōʻike antigen wikiwiki.Ua hoʻohana lākou i kahi ʻenehana i kapa ʻia ʻo deep mutation scanning e nānā like i ka hopena o kēlā me kēia mutation i loko o ka protein N o ka maʻi i ka hoʻopaʻa ʻana i kahi antibody diagnostic.Ua paʻi ʻia kā lākou hopena ma Cell ma Kepakemapa 15, 2022.
Ua hana nā mea noiʻi i kahi waihona piha o kahi kokoke i 8,000 N nā hoʻololi protein.ʻO kēia mau ʻano ʻokoʻa he ʻoi aku ma mua o 99.5% o nā hoʻololi āpau.A laila loiloi lākou i ke ʻano o ka launa pū ʻana o kēlā me kēia ʻano me 17 mau antibodies diagnostic like ʻole i hoʻohana ʻia i 11 mau hoʻokolohua antigen wikiwiki i loaʻa i ka pāʻoihana, me ka maʻamau.kit home.
Ua loiloi ka hui i nā mutations N-protein e pili ana i ka ʻike antibody.Ma muli o kēia ʻike, ua hana lākou i kahi "hoʻokele mutation profile" no kēlā me kēia antibody diagnostic.Hōʻike kēia ʻaoʻao i nā hoʻololi kikoʻī i loko o ka protein N i hiki ke hoʻopilikia i ka hiki o ka antibody ke hoʻopaʻa i kāna pahuhopu.Ua hōʻike ka hōʻike ʻana i nā antibodies i hoʻohana ʻia i nā hoʻokolohua wikiwiki i kēia lā e ʻike a hoʻopaʻa i nā ʻano like ʻole a pau o SARS-CoV-2 o ka hopohopo a me ka hopohopo.
ʻOiai ua ʻike kekahi mau antibodies diagnostic i ka ʻāpana like o ka protein N, ua ʻike nā mea noiʻi ua loaʻa i kēlā me kēia antibody kahi hōʻailona kūʻokoʻa o nā mutations pakele.Ke hoʻomau nei ka maʻi SARS-CoV-2 i ka mutate a hoʻoulu i nā ʻano hou, hiki ke hoʻohana ʻia kēia ʻikepili e hōʻailona i nā antibodies kit hōʻike e pono e loiloi hou ʻia.
"ʻO ka ʻike pololei a me ka maikaʻi o ka poʻe i maʻi ʻia he hoʻolālā koʻikoʻi no ka hoʻemi ʻana o COVID-19, a hāʻawi kā mākou noiʻi i ka ʻike e pili ana i nā mutations SARS-CoV-2 e hiki mai ana e hiki ke hoʻopilikia i ka ʻike," wahi a Ortlund."ʻO nā hopena i hōʻike ʻia ma aneʻi e ʻae iā mākou e hoʻololi koke i kēia maʻi ma muli o ka hoʻomau ʻana o nā ʻano hou, e hōʻike ana i nā hopena olakino a me ke olakino lehulehu."
ʻIke ʻia: ʻIke ʻo Mutation Deep Scan i nā mutations pakele i ka SARS-CoV-2 nucleocapsid me ka hoʻohana ʻana i nā hoʻokolohua antigen wikiwiki i kēia manawa.Frank F., Kin MM, Rao A., Bassit L., Liu H, Bowers HB, Patel AB, Kato ML, Sullivan JA, Greenleaf M., Piantadosi A., Lam VA, Hudson VH, Ortlund EA cell.2022 Kepakemapa 15;185(19):3603-3616.e13.Keena Kalaiaina: 10.1016/j.cell.2022.08.010.ʻAukake 29, 2022 PMID: 36084631.
Kākoʻo kālā: National Institute for Biomedical Imaging and Bioengineering NIH (NIBIB), National Institute of Diabetes, Digestive and Kidney Diseases (NIDDK) a me National Institute of Allergy and Infectious Diseases (NIAID), American Heart Association.
ʻO ka NIH Research Matters kahi hōʻano hou i kēlā me kēia pule o nā ʻike noiʻi NIH koʻikoʻi i loiloi ʻia e nā loea NIH.Ua paʻi ʻia e ka Communications and Public Affairs Office o ka Luna Hoʻokele o ka National Institutes of Health.