Hoohana Manao

ʻO ka Dengue NS1 Ag-IgG/IgM Combo Test he immunoassay chromatographic wikiwiki no ka ʻike qualitative o nā antibodies (IgG a me IgM) a me ka maʻi dengue NS1 antigen i ka maʻi dengue ma Serum a i ʻole Plasma e kōkua i ka hōʻoia ʻana o ka maʻi maʻi Dengue.

[SUMMER]

ʻO ka maʻi maʻi Dengue kahi maʻi maʻi lele i lawe ʻia e ka maʻi dengue i lawe ʻia e nā makika.ʻO ka maʻi maʻi Dengue hiki ke alakaʻi i ka maʻi recessive, dengue fever, dengue hemorrhagic fever, dengue hemorrhagic fever.ʻO nā hōʻike maʻamau o ka maʻi dengue ka hoʻomaka koke, ke kuni kiʻekiʻe, ke poʻo, ka ʻiʻo koʻikoʻi, ka iwi a me ka ʻeha ami, ka ʻili o ka ʻili, ke kahe koko, ka hoʻonui ʻana o ka lymph node, ka emi ʻana o ke koko keʻokeʻo helu, thrombocytopenia a pēlā aku i kekahi mau maʻi.Keia maʻi basically i loko o tropical a me ka subtropical wahi kaulana, no ka mea, ua laweia keia maʻi e Aides namu, kumu kaulana i kekahi seasonally, i loko o kēlā me kēia makahiki maʻamau i loko o Mei ~ Nowemapa, peak i loko o Iulai ~ Sepatemaba.Ma ka wahi hou maʻi ahulau, maʻa mau ka heluna kanaka, akā ʻo ka nui o ka maʻi maʻi maʻi, ma ka ʻāina endemic, ʻo ka nui o nā keiki.

Kumukānāwai

ʻO ka Dengue NS1 Ag-IgG/IgM Combo Test he immunoassay membrane qualitative e pili ana i ka immunoassay no ka ʻike ʻana i nā antibodies dengue virus (IgG a me IgM) a me dengue virus NS1 antigen ma Serum a i ʻole Plasma.

No ka ho'āʻo IgG/IgM: Aia ka mea hoʻāʻo: 1) kahi papa conjugate kala burgundy i loaʻa nā antigens envelopp recombinant dengue i hui pū ʻia me ke gula Colloid (dengue conjugates), 2) kahi ʻāpana nitrocellulose membrane i loaʻa nā laina hoʻāʻo ʻelua (laina T1 a me T2) a he laina mana (laina C).Ua uhi ʻia ka laina T1 me ka antibody no ka ʻike ʻana i ka IgM anti-dengue, ua uhi ʻia ka laina T2 me ka antibody no ka ʻike ʻana i ka IgG anti-dengue.Ke hāʻawi ʻia ka nui kūpono o ka mea hōʻike hōʻike i loko o ka pūnāwai laʻana o ka cassette hoʻāʻo, neʻe ka mea hōʻike ma ka hana capillary ma o ka cassette.IgG anti-dengue, inā aia i loko o ka specimen, e hoʻopaʻa i nā conjugates dengue.A laila hopu ʻia ka immunocomplex e ka reagent i uhi mua ʻia ma ka band T2, e hana ana i kahi laina T2 kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi dengue IgG a hōʻike i kahi maʻi hou a i ʻole hou.IgM anti-dengue inā loaʻa i loko o ka specimen e hoʻopaʻa i nā conjugates dengue.A laila hopu ʻia ka immunocomplex e ka reagent i uhi ʻia ma ka laina T1, e hana ana i kahi laina T1 kala burgundy, e hōʻike ana i kahi hopena hōʻike maikaʻi dengue IgM a hōʻike i kahi maʻi hou.ʻO ka loaʻa ʻole o nā laina T (T1 a me T2) hōʻike i kahi hopena maikaʻi ʻole.

No ka ho'āʻo NS1: Ma kēia kaʻina hana hoʻāʻo, hoʻopaʻa ʻia ka anti-Dengue NS1 antibody ma ka ʻāpana laina hoʻāʻo o ka cassette.Ma hope o ka hoʻokomo ʻia ʻana o kahi hōʻailona holoʻokoʻa / Serum / Plasma i loko o ka pūnāwai, hoʻopili ʻo ia me nā ʻāpana anti-Dengue NS1 i uhi ʻia i ka antibody i hoʻopili ʻia i ka pad specimen.Ke neʻe nei kēia hui ʻana i ka chromatographically ma ka lōʻihi o ka pahu hoʻāʻo a hui pū me ka anti-Dengue NS1 antibody immobilized.Inā loaʻa i ka mea hōʻailona ka antigen dengue virus NS1, e ʻike ʻia kahi laina kala ma ka ʻāpana laina hoʻāʻo e hōʻike ana i kahi hopena maikaʻi.Inā ʻaʻole i loaʻa i ka hōʻailona ka antigen dengue virus NS1, ʻaʻole e ʻike ʻia kahi laina kala ma kēia māhele e hōʻike ana i kahi hopena maikaʻi ʻole.

No ka lawelawe ʻana ma ke ʻano he mana kaʻina hana, e ʻike mau ʻia kahi laina kala ma ka ʻāpana laina mana e hōʻike ana ua hoʻohui ʻia ka nui kūpono o ka specimen a ua hana ʻia ka membrane wicking.

Ka Waihona a me ka Paʻa

E kūʻai e like me ka mea i hoʻopaʻa ʻia i loko o ka ʻeke i hoʻopaʻa ʻia i ka lumi wela a i ʻole i loko o ka friji (4-30 ℃ a i ʻole 40-86 ℉).Paʻa ka mea hoʻāʻo ma o ka lā pau i paʻi ʻia ma ka ʻeke i hoʻopaʻa ʻia.

Pono ka ho'āʻo i loko o ka ʻeke i hoʻopaʻa ʻia a hiki i ka hoʻohana ʻana.

ʻOhi ʻana a me ka hoʻomākaukau ʻana

1. Hiki ke hoʻohana ʻia ka Dengue NS1 Ag-IgG/IgM Combo Test ma Serum a i ʻole Plasma.

2. E hōʻiliʻili i ke koko holoʻokoʻa, serum a i ʻole plasma specimen ma hope o nā kaʻina hana hoʻokolohua maʻamau.

3. Pono e hana ʻia ka hoʻāʻo ma hope koke o ka hōʻiliʻili ʻana i nā mea hoʻohālike.Mai waiho i nā mea hoʻohālike ma ka lumi wela no nā manawa lōʻihi.No ka mālama lōʻihi, pono e mālama ʻia nā specimen ma lalo o -20 ℃.

4. E lawe mai i nā mea hoʻohālike i ka lumi wela ma mua o ka hoʻāʻo ʻana.Pono e hoʻoheheʻe ʻia a hui maikaʻi ʻia ma mua o ka hoʻāʻo ʻana.ʻAʻole pono e hoʻomaloʻo ʻia nā mea hoʻohālike a hoʻoheheʻe pinepine ʻia.

Kaʻina hana hoʻāʻo

E ʻae i ka hoʻāʻo, specimen, buffer a/a i ʻole nā ​​mana e hiki i ka lumi wela 15-30 ℃ (59-86℉) ma mua o ka hoʻāʻo.

1. E lawe mai i ka ʻeke i ka lumi wela ma mua o ka wehe ʻana.Wehe i ka mea hoʻāʻo mai ka ʻeke i hoʻopaʻa ʻia a hoʻohana koke i ka hiki.E kau i ka mea ho'āʻo ma kahi maʻemaʻe a pae.

2. No ka ho'āʻo IgG/IgM : E hoʻopaʻa i ka mea hoʻoheheʻe me ka pololei a hoʻololi i 1 kulu o ka specimen (ma kahi o 10μl) i ka pūnāwai specimen (S) o ka mea ho'āʻo, a laila e hoʻohui i 2 kulu o ka buffer (ma kahi o 70μl) a hoʻomaka i ka manawa.E nānā i ke kiʻi ma lalo.

3. No ka ho'āʻo NS1: E hoʻopaʻa i ka dropper vertically a hoʻololi i 8 ~ 10 kulu o ka serum a i ʻole plasma (ma kahi o 100μl) i ka pūnāwai specimen (S) o ka mea hoʻāʻo, a laila hoʻomaka i ka manawa.E nānā i ke kiʻi ma lalo.

4. E kali a puka mai ka laina kala.E heluhelu i nā hopena ma 15 mau minuke.Mai wehewehe i ka hopena ma hope o 20 mau minuke.

Ka wehewehe ʻana i nā hopena

Pono:

No ka ho'āʻo IgG/IgM: ʻIke ʻia ka laina hoʻokele a ma ka liʻiliʻi hoʻokahi laina hoʻāʻo ma ka membrane.ʻO ka hiʻohiʻona o ka laina hoʻāʻo T2 e hōʻike ana i ka loaʻa ʻana o nā antibodies IgG kikoʻī dengue.ʻO ka hiʻohiʻona o ka laina hoʻāʻo T1 e hōʻike ana i ka loaʻa ʻana o nā antibodies IgM kikoʻī dengue.A inā ʻike ʻia nā laina ʻelua T1 a me T2, hōʻike ia i ka loaʻa ʻana o nā antibodies IgG a me IgM kikoʻī dengue.ʻO ka haʻahaʻa o ka ʻike antibody, ʻoi aku ka nāwaliwali o ka laina hopena.

No ka ho'āʻo NS1: Hōʻike ʻia ʻelua laina.Pono e ʻike mau ʻia kekahi laina ma ka ʻāpana laina mana (C), a ʻo kekahi laina ʻokoʻa ʻē aʻe e ʻike ʻia ma ka ʻāpana laina hoʻāʻo.

ʻino:

Hoʻokahi laina kala i ʻike ʻia ma ka ʻāpana hoʻomalu (C).

He kūpono ʻole: ʻAʻole ʻike ʻia ka laina mana.ʻO ka lawa ʻole o ka nui o ka specimen a i ʻole nā ​​ʻenehana kaʻina hana hewa ke kumu nui loa o ka hemahema o ka laina hoʻokele.E nānā i ke kaʻina hana a hana hou i ka hoʻāʻo me kahi mea hoʻāʻo hou.Inā mau ka pilikia, e ho'opau koke i ka ho'ohana 'ana i ka pahu ho'ā'o a e ho'oka'a'ike i kāu mea ho'olaha kūloko.