Hoʻohui nā mea noiʻi Dutch i ka CRISPR a me ka bioluminescence i kahi hoʻokolohua hoʻokolohua nonā maʻi lele

Hiki i kahi protein nocturnal hou i hoʻomohala ʻia ke wikiwiki a hoʻomaʻamaʻa i ka ʻike o nā maʻi viral, e like me nā mea noiʻi ma Netherlands.
ʻO kā lākou aʻo ʻana, i paʻi ʻia i ka Pōʻakolu ma ACS Publications, e wehewehe ana i kahi ala maʻalahi, hoʻokahi ʻanuʻu no ka nānā wikiwiki ʻana i nā waikawa nucleic viral a me ko lākou helehelena me ka hoʻohana ʻana i nā protein uliuli a ʻōmaʻomaʻo paha.
ʻO ka ʻike ʻana i nā pathogens ma ka ʻike ʻana i kā lākou nucleic acid fingerprints he kumu koʻikoʻi i ka diagnostics clinical, biomedical research, a me ka nānā ʻana i ka meaʻai a me ke kaiapuni.ʻO nā ho'āʻo quantitative polymerase chain reaction (PCR) i hoʻohana nui ʻia he mea koʻikoʻi loa ia, akā koi ʻia ka hoʻomākaukau laʻana maʻalahi a i ʻole ka wehewehe ʻana i nā hopena, no laila ʻaʻole kūpono ia no kekahi mau hoʻonohonoho mālama olakino a i ʻole nā ​​​​kumu waiwai.
ʻO kēia pūʻulu mai Netherlands ka hopena o ka hui pū ʻana ma waena o nā ʻepekema mai nā kulanui a me nā halemai e hoʻomohala i kahi ala diagnostic nucleic acid wikiwiki, lawe a maʻalahi e hiki ke hoʻohana ʻia i nā ʻano hoʻonohonoho.
Ua hoʻoulu ʻia lākou e nā ʻālohilohi ahi, nā ʻālohilohi o ke ahi, a me nā hōkū liʻiliʻi o ka phytoplankton wai, nā mea i hoʻoikaika ʻia e kahi mea i kapa ʻia he bioluminescence.Hoʻokumu ʻia kēia hopena glow-in-the-dark e kahi hopena kemika e pili ana i ka protein luciferase.Ua hoʻokomo ka poʻe ʻepekema i nā protein luciferase i loko o nā mea ʻike e hoʻopuka i ka mālamalama e hoʻomaʻamaʻa i ka nānā ʻana ke loaʻa iā lākou kahi pahuhopu.ʻOiai ʻo kēia ka mea i kūpono i kēia mau mea ʻike no ka ʻike ʻana i ka point-of-care, ʻaʻole lākou i kēia manawa i ka ʻike kiʻekiʻe e pono ai no nā hoʻokolohua diagnostic clinical.ʻOiai hiki i ke ʻano hoʻoponopono gene CRISPR ke hāʻawi i kēia hiki, pono ia i nā ʻanuʻu he nui a me nā mea hana kūikawā hou e ʻike ai i ka hōʻailona nāwaliwali e hiki ke loaʻa i nā laʻana paʻakikī a walaʻau.
Ua loaʻa i nā mea noiʻi kahi ala e hoʻohui ai i kahi protein pili i ka CRISPR me kahi hōʻailona bioluminescent e hiki ke ʻike ʻia me kahi kiʻi kamepiula maʻalahi.No ka hōʻoia ʻana ua lawa ka RNA a i ʻole DNA no ka nānā ʻana, ua hana nā mea noiʻi i ka recombinase polymerase amplification (RPA), kahi ʻenehana maʻalahi e hana ana ma kahi mahana mau ma kahi o 100°F.Ua hoʻomohala lākou i kahi kahua hou i kapa ʻia ʻo Luminescent Nucleic Acid Sensor (LUNAS), kahi o nā protein CRISPR/Cas9 ʻelua i kikoʻī no nā ʻāpana pili like ʻole o ka genome viral, kēlā me kēia me kahi ʻāpana luciferase kū hoʻokahi i hoʻopili ʻia ma luna.
Ke hele mai nei ka genome viral kiko'ī a ka poʻe noiʻi e ʻimi nei, ʻelua mau protein CRISPR/Cas9 i hoʻopaʻa ʻia i ke kaʻina nucleic acid;lilo lākou i kahi kokoke, e ʻae ana i ka protein luciferase paʻa e hana a hoʻopuka i ke kukui polū i mua o kahi substrate kemika..No ka helu ʻana i ka substrate i hoʻopau ʻia i kēia kaʻina hana, ua hoʻohana nā mea noiʻi i ka hopena mana e hoʻokuʻu i ke kukui ʻōmaʻomaʻo.ʻO kahi paipu e hoʻololi i ka waihoʻoluʻu mai ka ʻōmaʻomaʻo a i ka uliuli e hōʻike ana i kahi hopena maikaʻi.
Ua hoʻāʻo ka poʻe noiʻi i kā lākou kahua ma ka hoʻomohala ʻana i ka assay RPA-LUNAS, kahi e ʻike aiRNA SARS-CoV-2me ka hoʻokaʻawale ʻole ʻana i ka RNA, a hōʻike i kāna hana diagnostic ma nā nasopharyngeal swab samples maiCOVID-19nā maʻi.Ua ʻike maikaʻi ʻia ʻo RPA-LUNAS iā SARS-CoV-2 i loko o 20 mau minuke i nā laʻana me kahi viral load RNA ma lalo o 200 kope/μL.
Manaʻo nā mea noiʻi hiki i kā lākou assay ke maʻalahi a ʻike pono i nā maʻi ʻē aʻe."He nani ka RPA-LUNAS no ka hoʻāʻo ʻana i ka maʻi maʻi maʻi maʻi," i kākau ai lākou.